Hepatocyte Spheroids & 3D Liver Models

Liver researchers use the 3D Petri Dish® to self-assemble hepatocyte spheroids — scaffold-free 3D liver models used in drug metabolism, hepatotoxicity, and liver disease research. Hepatic cells seeded into the non-adhesive agarose micro-mold settle into its recesses and form uniform spheroids through their own cell–cell adhesion, typically within 1–2 days, with no scaffold or animal-derived matrix.

Because each recess forms one spheroid of matched size, arrays of liver microtissues can be dosed and sampled consistently. The peer-reviewed publications below include primary hepatocyte and HepG2 spheroid studies, hepatotoxicity and fibrosis models, and 3D liver co-cultures built with the 3D Petri Dish®.