Summary
Published in Critical Reviews in Toxicology (2020), this peer-reviewed study used 3D Petri Dish® micro-molds to form scaffold-free 3D microtissues. Full citation: Zhang, Xihui, et al. Three-dimensional liver models: state of the art and their application for hepatotoxicity evaluation
Three-dimensional liver models: state of the art and their application for hepatotoxicity evaluation
Research Overview
Reconstructed human skin and cornea models are already written into regulatory testing guidelines, and 3D liver models are now used industrially for hepatotoxicity screening because they recapitulate the architecture, function, and toxicity responses of native liver.
This comprehensive review introduces both non-scaffold and scaffold-based methods for forming 3D liver models, weighing advantages and drawbacks of each, then examines the characteristics of primary human hepatocytes, stem-cell-derived hepatocyte-like cells, and immortalized liver lines as the cell sources feeding those models.
Key Discoveries
- Reviews scaffold-free and scaffold-based 3D liver model formation methods side by side
- Compares primary hepatocytes, stem-cell-derived hepatocyte-like cells, and immortalized lines
- Documents the hallmarks by which 3D liver models reproduce native liver responses
