Microtissues®
Distributed Worldwide by MilliporeSigma

The 3D Petri Dish®: Scaffold-Free 3D Cell Culture

The 3D Petri Dish® enables researchers to create hundreds of uniform spheroids and microtissues in a single pipetting step — no scaffold, no animal products, just cells.

457+
Peer-Reviewed Papers
20+
Cell Types Validated
100+
Universities Worldwide
81–256
Spheroids Per Well

Why Researchers Choose the 3D Petri Dish®

Uniform Spheroids

Hundreds of uniformly sized spheroids formed in one pipetting step for reproducible, high-throughput experiments.

Scaffold-Free

Cell-to-cell adhesion drives natural self-assembly. No synthetic scaffolds to alter cell physiology or confound results.

Reusable & Affordable

Autoclavable micro-molds fit standard 12- and 24-well dishes. Save thousands compared to consumable alternatives.

Imaging-Ready

Spheroids settle on the same optical plane for easy, high-content microscopy without repositioning.

Featured in Leading Journals

Selected highlights from over 457 publications in Nature, Science, Cell, and more.

Oncology

3D Bioprinted Breast Cancer‐Stroma Model with Tailored Migration‐Permissive Bioink Reveals Impact of Adipose‐Derived Stromal Cells on Cancer Cell Migration and Invasion Dynamics

Advanced Healthcare Materials · 2026

Published in Advanced Healthcare Materials (2026), this peer-reviewed study used 3D Petri Dish® micro-molds to form scaffold-free 3D microtissues. Full citation: Stecher, Sabrina, ...

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Neuroscience

3D cortical microtissue with innate microglia for studying real-time cell behavior across maturation and inflammatory response

bioRxiv · 2026

Published in bioRxiv (2026), this peer-reviewed study used 3D Petri Dish® micro-molds to form scaffold-free 3D microtissues. Full citation: Del Toro, Alexander, et al. 3D cortical ...

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Cardiovascular

A human cardiomyocyte-based cellular model mimicking cardiac ischemia

American Journal of Physiology-Cell Physiology · 2026

Published in American Journal of Physiology-Cell Physiology (2026), this peer-reviewed study used 3D Petri Dish® micro-molds to form scaffold-free 3D microtissues. Full citation: V...

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Tissue Engineering & Methods

A statistically rigorous multi-scale texture analysis framework for 3D spheroid characterization: temporal autocorrelation correction and molecular validation

Scientific Reports · 2026

Published in Scientific Reports (2026), this peer-reviewed study used 3D Petri Dish® micro-molds to form scaffold-free 3D microtissues. Full citation: Regassa, Daniel G., et al. A ...

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Trusted by Leading Institutions

Used in peer-reviewed research at over 100 universities and medical centers worldwide.

Columbia

University

MIT

Massachusetts Institute of Technology

Brown

University

Baylor

College of Medicine

Harvard

University

The 3D Cell Culture Technology

The 3D Petri Dish® is the natural 3D cell culture environment that is scaffold-free and maximizes cell-to-cell interactions. When cells are seeded onto the 3D Petri Dish®, cell-to-cell adhesion drives self-assembly of the 3D microtissue, typically in 1–2 days.

  • Make hundreds of spheroids of uniform size in a single pipetting step
  • Control spheroid size precisely
  • Image an array of spheroids on the same optical plane
  • Form spheroids of two different cell types
  • Grow spheroids from single cell clones
  • Harvest spheroids for RT-PCR and Western Blots without enzymes
  • Form microtissues in complex shapes for novel applications
View Protocols
3D Petri Dish® micro-mold (12-256)

Ready to Go 3D?

Join researchers at 100+ institutions who have made the switch to scaffold-free 3D cell culture.

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