Cardiac Microtissues & Heart Spheroids

Cardiovascular labs use the 3D Petri Dish® to self-assemble cardiac microtissues — scaffold-free 3D cultures of cardiomyocytes, cardiac fibroblasts, and endothelial cells that recover the cell–cell contacts flat culture can’t provide. Cells seeded into the non-adhesive agarose micro-mold settle into its recesses and, driven by their own adhesion, form uniform 3D microtissues, typically within 1–2 days.

Because every recess produces a matched aggregate, arrays of cardiac microtissues can be dosed and imaged consistently — the studies below include iPSC-derived cardiomyopathy models, cardiotoxicity screening, engineered heart-repair constructs, and cardiac co-culture work, all built on the 3D Petri Dish®.

Getting started: follow the step-by-step protocols for casting, seeding, and harvesting, or browse 3D Petri Dish® micro-molds for 12- and 24-well plates — $575/pack, free worldwide shipping.

Cardiac Microtissues & Heart Spheroids

34 peer-reviewed publications

A human cardiomyocyte-based cellular model mimicking cardiac ischemia

Computationally informed point of departure evaluation for proarrhythmic cardiotoxicity assessment using 3D engineered cardiac microtissues from human iPSC-derived cardiomyocytes

Generation and applications of cardiac spheroids

Lab-grown, 3D extracellular matrix particles improve cardiac function and morphology in myocardial ischemia

Multiscale profiling of tyrosine kinase inhibitor cardiotoxicity reveals mechanosensitive ion channel PIEZO1 as cardioprotective

In vitro to in vivo extrapolation from 3D hiPSC-derived cardiac microtissues and physiologically based pharmacokinetic modeling to inform next-generation arrhythmia risk assessment

Proximal telomeric decompaction due to telomere shortening drives FOXC1-dependent myocardial senescence

Toxicity of low dose bisphenols in human iPSC-derived cardiomyocytes and human cardiac organoids – Impact on contractile function and hypertrophy

Vascular units as advanced living materials for bottom-up engineering of perfusable 3D microvascular networks

Action potential metrics and automated data analysis pipeline for cardiotoxicity testing using optically mapped hiPSC-derived 3D cardiac microtissues

Adult Multipotent Cardiac Progenitor-Derived Spheroids: A Reproducible Model of In Vitro Cardiomyocyte Commitment and Specification

Applied Electric Fields Polarize Initiation and Growth of Endothelial Sprouts

Correction of DMD in human iPSC-derived cardiomyocytes by base-editing-induced exon skipping

Nanowired human cardiac organoid transplantation enables highly efficient and effective recovery of infarcted hearts

Proarrhythmic toxicity of low dose bisphenol A and its analogs in human iPSC-derived cardiomyocytes and human cardiac organoids through delay of cardiac repolarization

Arrhythmia Assessment in Heterotypic Human Cardiac Myocyte–Fibroblast Microtissues

A predictive in vitro risk assessment platform for pro-arrhythmic toxicity using human 3D cardiac microtissues

DYNAMIC BIOREACTOR MODEL TO MIMIC EARLY CARDIAC FIBROSIS IN DIABETES

Fabrication of 3D Cardiac Microtissue Arrays using Human iPSC-Derived Cardiomyocytes, Cardiac Fibroblasts, and Endothelial Cells

Human Atrial Cardiac Microtissues for Chamber-Specific Arrhythmic Risk Assessment

Multicellular Human Cardiac Organoids Transcriptomically Model Distinct Tissue-Level Features of Adult Myocardium

Pathogenic LMNA variants disrupt cardiac lamina-chromatin interactions and de-repress alternative fate genes

Human cardiac organoids for the modelling of myocardial infarction and drug cardiotoxicity

Modeling Secondary Iron Overload Cardiomyopathy with Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Targeting HIF‐α for robust prevascularization of human cardiac organoids

3D Cardiac Cell Culture: A Critical Review of Current Technologies and Applications

Directed fusion of cardiac spheroids into larger heterocellular microtissues enables investigation of cardiac action potential propagation via cardiac fibroblasts

Soluble VEGFR1 signaling guides vascular patterns into dense branching morphologies

Cell number per spheroid and electrical conductivity of nanowires influence the function of silicon nanowired human cardiac spheroids

Co‐Differentiation of Human Pluripotent Stem Cells‐Derived Cardiomyocytes and Endothelial Cells from Cardiac Mesoderm Provides a Three‐Dimensional Model of Cardiac Microtissue

Gq-activated fibroblasts induce cardiomyocyte action potential prolongation and automaticity in a three-dimensional microtissue environment

Inspiration from heart development: Biomimetic development of functional human cardiac organoids

Intracoronary Delivery of Self-Assembling Heart-Derived Microtissues (Cardiospheres) for Prevention of Adverse Remodeling in a Pig Model of Convalescent Myocardial Infarction

Functional scaffold-free 3-D cardiac microtissues: a novel model for the investigation of heart cells

Frequently asked questions

Which cardiac cell types form microtissues in the 3D Petri Dish®?

Can I make cardiac spheroids of a specific size?

Can different cardiac cell types be combined in one microtissue?

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