Summary
Published in Cellular and Molecular Bioengineering (2021), this peer-reviewed study used 3D Petri Dish® micro-molds to form scaffold-free 3D microtissues. Full citation: Soepriatna, Arvin H., et al. Human Atrial Cardiac Microtissues for Chamber-Specific Arrhythmic Risk Assessment
Human Atrial Cardiac Microtissues for Chamber-Specific Arrhythmic Risk Assessment
Research Overview
Atrial fibrillation is the most prevalent electrical conduction disorder, yet the mechanisms behind atrial arrhythmias remain elusive — partly because most cardiac models use ventricular cells.
This study developed a robust in vitro model of 3D atrial microtissue from human iPSC-derived cardiomyocytes, evaluating chamber-specific chemical responses experimentally and computationally. Atrial and ventricular cardiomyocytes were differentiated from GCaMP6f-expressing iPSCs, action potential activity assessed by fluorescence imaging, and self-assembling microtissues formed from lactate-purified cardiomyocytes with 5% human cardiac fibroblasts, then electrically stimulated.
Key Discoveries
- Chamber-specific 3D atrial microtissues distinguished from ventricular models
- Self-assembled from lactate-purified iPSC-cardiomyocytes with 5% cardiac fibroblasts
- GCaMP6f fluorescence imaging plus computational modeling assessed arrhythmic risk
