Summary
Published in The Prostate (2018), this peer-reviewed study used 3D Petri Dish® micro-molds to form scaffold-free 3D microtissues. Full citation: Ojalill, Marjaana, et al. The composition of prostate core matrisome in vivo and in vitro unveiled by mass spectrometric analysis
The composition of prostate core matrisome in vivo and in vitro unveiled by mass spectrometric analysis
Research Overview
Extracellular matrix composition and organization regulate cell behavior, and in the prostate this is underscored by the fact that several candidate prostate stem cell markers are matrix receptors.
This study established 12 fibroblastic cell lines from cancerous and non-cancerous areas of six prostates, let the cells produce matrix in culture, and performed proteome-wide mass spectrometry of the matrix components. Comparing the matrix made in culture by six non-cancerous-tissue-derived fibroblasts against the in vivo matrisome allowed the authors to characterize how fibroblastic cells become activated in vitro.
Key Discoveries
- 12 fibroblastic lines established from cancerous and non-cancerous regions of six prostates
- Proteome-wide mass spectrometry characterized the cell-produced core matrisome
- Cultured matrix compared against the in vivo matrisome to reveal in vitro fibroblast activation
