Summary
Published in Pharmaceutical Research (2014), this peer-reviewed study used 3D Petri Dish® micro-molds to form scaffold-free 3D microtissues. Full citation: Deo, Vipin Kumar, et al. A Model for Targeting Colon Carcinoma Cells Using Single-Chain Variable Fragments Anchored on Virus-Like Particles via Glycosyl Phosphatidylinositol Anchor
A Model for Targeting Colon Carcinoma Cells Using Single-Chain Variable Fragments Anchored on Virus-Like Particles via Glycosyl Phosphatidylinositol Anchor
Research Overview
Virus-like particles displaying tumor-targeting single-chain variable fragments against tumor-associated glycoprotein-72 have potential for colon carcinoma immunotherapy. In this study, the fragments were anchored on virus-like particles via glycosylphosphatidylinositol to target colon carcinoma spheroids in vitro.
The particles were produced by co-injecting two Bombyx mori nucleopolyhedrovirus bacmids into silkworm larvae. Large unilamellar vesicles 100 nm in diameter, made from DOPC and packaged with Sulforhodamine B, were used to assess association and delivery — testing targeted delivery against 3D tumor spheroids rather than monolayers.
Key Discoveries
- Virus-like particles displaying anti-TAG-72 antibody fragments produced in silkworm larvae
- GPI anchoring presented the targeting fragments on the particle surface
- Targeting and delivery evaluated against colon carcinoma spheroids in 3D
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Frequently Asked Questions
Why use tumor spheroids for cancer research?Can 3D spheroids be used for drug screening?
Yes, 3D tumor spheroids created with 3D Petri Dish® micro-molds are widely used for high-throughput drug screening. The uniform size and reproducibility of the spheroids enables reliable dose-response measurements and comparison across experimental conditions.