Scaffold-Free Endometrial Organoids Respond to Excess Androgens Associated With Polycystic Ovarian Syndrome

Research Overview

Polycystic ovary syndrome affects many women of reproductive age and raises the risk of endometrial cancer, but the effect of its characteristic elevated androgens on the endometrium is hard to study.

This study established a scaffold-free multicellular endometrial organoid built from primary endometrial epithelial and stromal cells. Organoids were treated for 14 days with physiologic levels of estradiol and testosterone reproducing either a normal follicular phase or a PCOS hormone profile, then harvested for immunostaining and RNA sequencing — isolating the endometrial response to androgen excess.