Summary
Published in Cytotechnology (2021), this peer-reviewed study used 3D Petri Dish® micro-molds to form scaffold-free 3D microtissues. Full citation: Yildiz-Ozturk, Ece, et al. Lung carcinoma spheroids embedded in a microfluidic platform
🧬 Oncology
Lung carcinoma spheroids embedded in a microfluidic platform
Cytotechnology 2021 Yildiz-Ozturk, Ece, et al
Cite as: Yildiz-Ozturk, Ece, et al. Lung carcinoma spheroids embedded in a microfluidic platform. Cytotechnology (2021). doi:10.1007/s10616-021-00470-7 doi.org/10.1007/s10616-021-00470-7
Research Overview
This study developed a lung carcinoma spheroid platform with microfluidic perfusion to mimic lung cancer pathology and test the candidate drug panaxatriol.
Spheroids formed on agar microtissue molds over 10 days, reaching an average diameter of 317.18 ± 4.05 µm, and were then transferred into a dynamic microfluidic system with perfusion. Embedded in Matrigel within the platform, the spheroids continued to grow — combining scaffold-free spheroid formation with perfused, flow-based culture for drug evaluation.
Key Discoveries
- Lung carcinoma spheroids formed on agar molds to 317 ± 4 µm over 10 days
- Transferred into a perfused microfluidic platform embedded in Matrigel
- Used to evaluate the candidate drug molecule panaxatriol under dynamic flow
