Summary
Published in OncoTargets and Therapy (2022), this peer-reviewed study used 3D Petri Dish® micro-molds to form scaffold-free 3D microtissues. Full citation: Duś-Szachniewicz, Kamila, et al. Development and Characterization of 3D Hybrid Spheroids for the Investigation of the Crosstalk Between B-Cell Non-Hodgkin Lymphomas and Mesenchymal Stromal Cells
Development and Characterization of 3D Hybrid Spheroids for the Investigation of the Crosstalk Between B-Cell Non-Hodgkin Lymphomas and Mesenchymal Stromal Cells
Research Overview
In aggressive B-cell non-Hodgkin lymphomas, bone marrow involvement is hard to treat partly because lymphoma cells shelter behind protective interactions with mesenchymal stromal cells — a relationship poorly characterized for lack of reproducible 3D models.
This study developed size-controlled, stable hybrid spheroids combining Ri-1 (diffuse large B-cell lymphoma) or RAJI (Burkitt lymphoma) cells with HS-5 stromal fibroblasts, using commercially available agarose hydrogel microwells for a fast, low-cost, reproducible format. The result is a practical platform for studying lymphoma-stroma crosstalk and testing therapies against the protected niche.
Key Discoveries
- Size-controlled hybrid lymphoma/stromal spheroids formed in agarose hydrogel microwells
- Models both diffuse large B-cell (Ri-1) and Burkitt (RAJI) lymphoma with HS-5 stroma
- Fast, low-cost, reproducible platform for studying protective lymphoma-MSC crosstalk
