Summary
Research published in Nature Biotechnology (2023) created a comprehensive developmental atlas of human retinal organoids using 3D Petri Dish®. The 'Laminator' algorithm enables standardized analysis of retinal layer formation from iPSCs.
Multimodal spatiotemporal phenotyping of human retinal organoid development
Research Overview
Organoids from human pluripotent stem cells enable study of development and disease, but quantitative measurement across spatial scales and molecular modalities has been missing. This study generated multiplexed protein maps across a retinal organoid time course alongside primary adult human retinal tissue.
The team built a toolkit to visualize progenitor and neuron location, spatial arrangement of extracellular and subcellular components, and global patterning in both organoids and primary tissue. They also generated single-cell transcriptome and chromatin accessibility time-course data, inferred the gene regulatory network underlying organoid development, and integrated genomic with spatially resolved data.
Key Discoveries
- Created comprehensive atlas of retinal organoid development over time
- Developed 'Laminator' algorithm for automated layer analysis
- Identified key developmental milestones and timing
- Established standardized protocols for retinal organoid generation
3D Petri Dish® Application
Enabled standardized generation of retinal organoids from iPSCs with consistent size and quality
- iPSC Aggregation: Uniform embryoid body formation for consistent differentiation
- Standardization: Reproducible organoid size enabled quantitative analysis
- High-Throughput: Generated sufficient organoids for comprehensive phenotyping
Recommended Products
12-256 Small Spheroids
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12-81 Large Spheroids
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24-35 Large Spheroids
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Frequently Asked Questions
How are retinal organoids made from stem cells?
Retinal organoids are generated by first aggregating iPSCs into uniform embryoid bodies using 3D Petri Dish® micro-molds, then guiding differentiation with specific growth factors over several months.
What is the Laminator algorithm for retinal organoids?
Laminator is a computational tool developed in this study that automatically identifies and quantifies retinal layers in organoids, enabling standardized quality assessment and comparison across experiments.
Which 3D Petri Dish® product is best for organoid research?
Start with the 12-256 Small Spheroid Kit for iPSC aggregation, then transfer to 12-81 Large Spheroid Kit for organoid maturation and long-term culture.