Organ-on-e-chip: Three-dimensional self-rolled biosensor array for electrical interrogations of human electrogenic spheroids

Research Overview

Cell-cell communication coordinates biological systems, and 3D spheroids offer a venue to study it because their architecture mimics the native microenvironment. But electrophysiology — the main tool for electroactive cells — has lacked direct, multisite, simultaneous access to tissue in three dimensions.

This study implemented 3D self-rolled biosensor arrays, built as either active field-effect transistors or passive microelectrodes, to interface human cardiac spheroids in 3D. The arrays delivered continuous, stable, multiplexed recordings of field potentials — bringing genuine 3D electrophysiology to cardiac microtissues.