Summary
Published in Cells (2024), this publication cites the 3D Petri Dish® micro-mold system rather than reporting its own experimental use of it. Full citation: Gómez-Mercader, Antonio, et al. Fish Cell Spheroids, a Promising In Vitro Model to Mimic In Vivo Research: A Review
Fish Cell Spheroids, a Promising In Vitro Model to Mimic In Vivo Research: A Review
Research Overview
In vitro systems let researchers dissect cellular mechanisms in a controlled setting while sidestepping the ethical and logistical burdens of animal work, and 3D culture — spheroids in particular — has become the most straightforward way to approximate in vivo conditions. In fish biology, however, 3D culture has been used sparingly, mostly for physiological and morphological characterization.
This review surveys the emerging applications of fish cell spheroids, arguing that their ability to emulate in vivo conditions makes them a promising in vitro model for aquaculture, toxicology, and comparative biology research.
Key Discoveries
- Reviews the limited but growing use of 3D spheroid culture with fish cells
- Positions spheroids as the most accessible 3D alternative to fish monolayer cultures
- Identifies applications beyond morphology, including physiology and toxicology
