Summary
Published in The European Physical Journal E (2010), this peer-reviewed study used 3D Petri Dish® micro-molds to form scaffold-free 3D microtissues. Full citation: Klopper, A. V., et al. Finite-size corrections to scaling behavior in sorted cell aggregates
Finite-size corrections to scaling behavior in sorted cell aggregates
Research Overview
Cell sorting is pivotal to early development of multicellular organisms, and in vitro studies have revealed the cellular properties that drive it — but those studies have been limited to two-dimensional analysis of what is fundamentally a three-dimensional process.
This study describes a method to record sorting of primary zebrafish ectoderm and mesoderm germ layer progenitor cells in three dimensions over time, analyzing behavior quantitatively with an order parameter based on heterotypic interface length, and investigating how sorting outcomes depend on cell population size.
Key Discoveries
- Method records germ layer progenitor cell sorting in three dimensions over time
- Order parameter based on heterotypic interface length quantified sorting progress
- Population size dependence of sorted aggregates investigated with finite-size corrections
