Summary
Published in Lab on a Chip (2021), this peer-reviewed study used 3D Petri Dish® micro-molds to form scaffold-free 3D microtissues. Full citation: Sevetson, Jessica L., et al. Cortical spheroids display oscillatory network dynamics
🧠 Neuroscience
Cortical spheroids display oscillatory network dynamics
Lab on a Chip 2021 Sevetson, Jessica L., et al
Cite as: Sevetson, Jessica L., et al. Cortical spheroids display oscillatory network dynamics. Lab on a Chip (2021). doi:10.1039/D1LC00737H doi.org/10.1039/D1LC00737H
Research Overview
This work demonstrated that 3D cortical spheroid cultures do not merely contain neurons — they build functioning networks. Networks developed by day 9 and produced increasingly complex activity patterns as the cultures matured.
Crucially, the authors showed for the first time that spheroids exhibit network activity resembling in vivo network events, establishing scaffold-free cortical spheroids as a platform for studying oscillatory brain network dynamics in vitro.
Key Discoveries
- Functional neural networks developed in cortical spheroids by day 9 in vitro
- Network activity patterns grew more complex as cultures matured
- First demonstration of spheroid network events resembling in vivo brain activity
