Summary
Published in Scientific Data (2025), this peer-reviewed study used 3D Petri Dish® micro-molds to form scaffold-free 3D microtissues. Full citation: Blondeel, Eva, et al. The Spheroid Light Microscopy Image Atlas for morphometrical analysis of three-dimensional cell cultures
The Spheroid Light Microscopy Image Atlas for morphometrical analysis of three-dimensional cell cultures
Research Overview
3D cultures are increasingly popular in academia and industry, yet the morphology of the 3D architecture itself remains remarkably understudied. This work introduced the open-access Spheroid Light Microscopy Image Atlas (SLiMIA) as a training set for morphology studies.
The dataset contains roughly 8,000 spheroid images with extensive metadata spanning 9 microscopes, 47 cell lines, 8 culture media, 4 spheroid formation methods, and multiple seeding densities — a resource to guide spheroid researchers and economize resources by advancing 3D culture optimization and standardization.
Key Discoveries
- Open-access atlas of ~8,000 spheroid light microscopy images with rich metadata
- Spans 9 microscopes, 47 cell lines, 8 media, and 4 spheroid formation methods
- Provides a training set for computational morphology studies of 3D cultures
