Summary
Published in BMC Oral Health (2019), this peer-reviewed study used 3D Petri Dish® micro-molds to form scaffold-free 3D microtissues. Full citation: Janjić, Klara, et al. The impact of collagen membranes on 3D gingival fibroblast toroids
The impact of collagen membranes on 3D gingival fibroblast toroids
Research Overview
Guided tissue regeneration depends on biomaterial testing, and 3D cell constructs offer a way to bridge cell culture and animal models. Hypothesizing that cell attachment behavior could be observed in toroidal 3D constructs, this study evaluated 3D gingival fibroblast toroids as a simple in vitro assay for attachment of oral fibroblasts to collagen membranes.
Ring-shaped toroids were formed from human gingival fibroblasts, checked by hematoxylin-eosin staining, and seeded onto plastic surfaces or collagen membranes. Morphology was documented at 24, 48, and 72 hours by light and fluorescence microscopy, and toroid vitality was measured at the same time points with a resazurin-based assay.
Key Discoveries
- Gingival fibroblast toroids proposed as a simple attachment assay for collagen membranes
- Toroids seeded on plastic versus collagen membrane and followed at 24, 48, and 72 h
- Morphology by light/fluorescence microscopy with resazurin vitality readout
