Summary
Published in Clinical Oral Investigations (2020), this peer-reviewed study used 3D Petri Dish® micro-molds to form scaffold-free 3D microtissues. Full citation: Oberoi, Gunpreet, et al. The impact of 3D-printed LAY-FOMM 40 and LAY-FOMM 60 on L929 cells and human oral fibroblasts
The impact of 3D-printed LAY-FOMM 40 and LAY-FOMM 60 on L929 cells and human oral fibroblasts
Research Overview
LAY-FOMM is a promising material for FDA-approved fused deposition modeling applications in drug delivery, so its effect on oral cells matters. This study evaluated 3D-printed LAY-FOMM 40, LAY-FOMM 60, and biocompatible polylactic acid against murine L929 cells, gingival fibroblasts, and periodontal ligament fibroblasts.
Testing spanned indirect exposure, direct monolayer culture, and direct spheroid culture, using resazurin-based toxicity assays confirmed by MTT and live/dead staining, with surface topography examined by scanning electron microscopy. Both LAY-FOMM formulations reduced resazurin conversion, indicating a measurable impact on oral cell activity.
Key Discoveries
- LAY-FOMM 40 and 60 compared against polylactic acid for oral cell biocompatibility
- Tested in indirect, direct monolayer, and direct spheroid culture models
- Both LAY-FOMM materials reduced cell activity; surface topography examined by SEM
