Summary
Published in Biomedical Optics Express (2024), this peer-reviewed study used 3D Petri Dish® micro-molds to form scaffold-free 3D microtissues. Full citation: Chen, Keyu, et al. Line-field dynamic optical coherence tomography platform for volumetric assessment of biological tissues
Line-field dynamic optical coherence tomography platform for volumetric assessment of biological tissues
Research Overview
Dynamic optical coherence tomography uses time-dependent signal fluctuations to add contrast and probe cellular physiology, but most published work is limited to 2D images because acquisition rates constrain volumetric imaging.
This study introduced a high-speed spectral-domain line-field dynamic OCT system with acquisition protocols designed for fast volumetric imaging of biological tissue. The probe uses an exchangeable afocal lens pair, letting users select transverse resolution from 1.1 µm to 6.4 µm — label-free volumetric imaging suited to 3D cultures and microtissues.
Key Discoveries
- Line-field spectral-domain dynamic OCT enabled fast volumetric rather than 2D imaging
- Exchangeable afocal lens pair provided selectable transverse resolution from 1.1 to 6.4 µm
- Label-free contrast derived from time-dependent intracellular signal fluctuations
