Summary
Published in International Journal of Molecular Sciences (2020), this peer-reviewed study used 3D Petri Dish® micro-molds to form scaffold-free 3D microtissues. Full citation: Silva, Daniel N., et al. Influence of ClearT and ClearT2 Agitation Conditions in the Fluorescence Imaging of 3D Spheroids
Influence of ClearT and ClearT2 Agitation Conditions in the Fluorescence Imaging of 3D Spheroids
Research Overview
Tumor spheroids are powerful screening tools, but confocal imaging of them is limited by how little light penetrates thick samples — undermining reproducibility of structural and therapeutic readouts. Optical clearing solutions increase transparency by reducing light scattering.
This study examined ClearT and ClearT2 clearing protocols, including the influence of agitation, to optimize spheroid transparency for confocal laser scanning microscopy — practical method development for getting reliable, deep images of 3D tumor models used in anticancer screening.
Key Discoveries
- ClearT and ClearT2 optical clearing evaluated for tumor spheroid transparency
- Agitation examined as a variable in clearing effectiveness
- Targets the light-penetration limit that constrains confocal imaging of thick spheroids
