Summary
Published in Cells (2022), this peer-reviewed study used 3D Petri Dish® micro-molds to form scaffold-free 3D microtissues. Full citation: Duś-Szachniewicz, Kamila, et al. Formation of Lymphoma Hybrid Spheroids and Drug Testing in Real Time with the Use of Fluorescence Optical Tweezers
Formation of Lymphoma Hybrid Spheroids and Drug Testing in Real Time with the Use of Fluorescence Optical Tweezers
Research Overview
Stromal–lymphoma interactions in the bone marrow drive drug resistance and therapy failure, yet physiologically relevant 3D models of that microenvironment did not exist. This study proposed optically controlled assembly of hybrid lymphoma spheroids using optical tweezers.
Stromal spheroids were first prepared in agarose hydrogel; then two aggressive B-cell lymphoma lines — Ri-1 (diffuse large B-cell) and Raji (Burkitt) — were guided onto them with in-house-built fluorescence optical tweezers to form multicellular hybrids. The hybrids held their 3D architecture for 24 hours and were used to evaluate anticancer agents including doxorubicin and ibrutinib.
Key Discoveries
- Optical tweezers assembled lymphoma cells onto stromal spheroids formed in agarose hydrogel
- Ri-1 (DLBCL) and Raji (Burkitt) hybrids preserved 3D architecture for 24 h
- Model used to evaluate doxorubicin and ibrutinib against stroma-protected lymphoma
