Summary
Published in Sensors (2022), this peer-reviewed study used 3D Petri Dish® micro-molds to form scaffold-free 3D microtissues. Full citation: Calabretta, Maria Maddalena, et al. Bioluminescence Sensing in 3D Spherical Microtissues for Multiple Bioactivity Analysis of Environmental Samples
Bioluminescence Sensing in 3D Spherical Microtissues for Multiple Bioactivity Analysis of Environmental Samples
Research Overview
Rapid sensing of a sample’s diverse bioactivities matters both for monitoring environmental toxicants and for understanding how they act on molecular pathways — knowledge central to hazard characterization and risk assessment of chemicals, surface waters, and effluents.
The authors developed and optimized a straightforward multiplexed bioluminescent assay that measures four toxicologically relevant activities in bioluminescent 3D microtissues: inflammatory activity, antioxidant activity, toxicity, and the presence of heavy metals. Applied to river water samples, the assay demonstrated its potential for environmental screening.
Key Discoveries
- Multiplexed bioluminescent assay reads four bioactivities in 3D microtissues
- Detects inflammatory, antioxidant, toxic activity, and heavy metals in one platform
- Successfully applied to river water samples
