Summary
Published in Multiphoton Microscopy in the Biomedical Sciences XX (2020), this peer-reviewed study used 3D Petri Dish® micro-molds to form scaffold-free 3D microtissues. Full citation: Garcia, Edwin, et al. FLIM, FRET and high content analysis
FLIM, FRET and high content analysis
Research Overview
Fluorescence lifetime imaging (FLIM) distinguishes molecular species and maps variations in a fluorophore’s local molecular environment, including reading out Förster resonant energy transfer (FRET) to assay protein interactions or genetically expressed biosensors.
This work implemented wide-field time-gated FLIM in a modular, open, automated microscopy platform for high-content analysis. To show relevance to drug discovery, the openFLIM platform assayed interactions of low-copy-number endogenous proteins in yeast cells labelled with fluorescent proteins — establishing that lifetime-based readouts can run at screening scale rather than one sample at a time.
Key Discoveries
- Wide-field time-gated FLIM implemented in an open, automated high-content analysis platform
- Assayed interactions of low-copy-number endogenous proteins in labelled yeast cells
- Brings FRET-based protein interaction readouts to drug-discovery-scale screening
