Summary
Published in BioMed Research International (2017), this peer-reviewed study used 3D Petri Dish® micro-molds to form scaffold-free 3D microtissues. Full citation: Kurzmann, Christoph, et al. Evaluation of Resins for Stereolithographic 3D-Printed Surgical Guides: The Response of L929 Cells and Human Gingival Fibroblasts
Evaluation of Resins for Stereolithographic 3D-Printed Surgical Guides: The Response of L929 Cells and Human Gingival Fibroblasts
Research Overview
Desktop stereolithography printers have made 3D-printed surgical guides accessible to dental offices, raising the question of how the printing resins affect the soft tissue they contact. This study tested Clear and Dental SG resins at different stages of processing against L929 cells and human gingival fibroblasts.
Cell activity was assessed in indirect and direct monolayer models plus a direct spheroid culture model, using MTT and resazurin toxicity assays with live/dead staining. The overall finding was that biological impact depends on the resin’s processing stage — post-processing matters as much as material choice for dental biocompatibility.
Key Discoveries
- Clear and Dental SG SLA resins tested against L929 cells and gingival fibroblasts
- Assessed in indirect, direct monolayer, and direct spheroid culture models
- Cytotoxic impact depended on the processing stage of the printed material
