Summary
Published in Toxicology Letters (2025), this study establishes a two-chamber liver-organ co-culture system in a higher-throughput 96-well format for evaluating toxicity in the presence of physiological hepatic metabolism. Using 3D Petri Dish® micro-molds to generate uniform hepatic microtissues, the platform enables assessment of androgenic responses from endocrine-disrupting chemicals (EDCs) while maintaining metabolic competence.
🔬 Other
Establishing scientific confidence in a two-chamber co-culture system to evaluate androgenic response in the presence of hepatic metabolism
Toxicology Letters, 2025 · van Tongeren, T.C.A. et al 2025 van Tongeren, T.C.A. et al
Cite as: Citation: van Tongeren, T.C.A. et al. Establishing scientific confidence in a two-chamber co-culture system to evaluate androgenic response in the presence of hepatic metabolism. Toxicology Letters 2025 doi.org/10.1016/j.toxlet.2025.08.014
Key Discoveries
- Higher-throughput toxicity screening — The two-chamber co-culture model was established in a 96-well format, significantly increasing throughput for endocrine-disrupting chemical (EDC) risk assessment compared to existing approaches.
- Maintained hepatic metabolism — The system successfully maintains hepatic function throughout the co-culture period, enabling target tissue assessment in the presence of physiologically relevant drug metabolism.
- Physiological prostate-liver crosstalk — The 3D prostate models reflect physiologically relevant prostate epithelial and stromal crosstalk, advancing current androgen assessment beyond traditional 2D screening methods.
- Validated for androgenic response — The platform was developed and validated for evaluating androgenic responses, demonstrating reliable assessment of endocrine-disrupting potential with metabolic competence.
3D Petri Dish® Application
3D Petri Dish®