Summary
Published in BIO-PROTOCOL (2022), this peer-reviewed study used 3D Petri Dish® micro-molds to form scaffold-free 3D microtissues. Full citation: Van Zundert, Indra, et al. Fluorescence Imaging of 3D Cell Models with Subcellular Resolution
🧪 Tissue Engineering & Methods
Fluorescence Imaging of 3D Cell Models with Subcellular Resolution
BIO-PROTOCOL 2022 Van Zundert, Indra, et al
Cite as: Van Zundert, Indra, et al. Fluorescence Imaging of 3D Cell Models with Subcellular Resolution. BIO-PROTOCOL (2022). doi:10.21769/BioProtoc.4469 doi.org/10.21769/BioProtoc.4469
Research Overview
This protocol paper addresses a practical bottleneck in 3D culture: getting clear images of intact organoids and spheroids. It provides detailed guidelines for imaging 3D cell models by confocal and multi-photon fluorescence microscopy.
Following the described procedures, images of 3D culture systems can be acquired with sub-cellular resolution — allowing structures inside intact spheroids and organoids to be resolved without sectioning.
Key Discoveries
- Step-by-step imaging guidelines for organoids and spheroids by confocal multi-photon microscopy
- Sub-cellular resolution achievable in intact 3D cell models
- Reduces reliance on physical sectioning for interior visualization
