Summary
Published in Frontiers in Physiology (2018), this peer-reviewed study used 3D Petri Dish® micro-molds to form scaffold-free 3D microtissues. Full citation: Oberoi, Gunpreet, et al. Contraction Dynamics of Rod Microtissues of Gingiva-Derived and Periodontal Ligament-Derived Cells
Contraction Dynamics of Rod Microtissues of Gingiva-Derived and Periodontal Ligament-Derived Cells
Research Overview
Directed self-assembly of microtissues could underpin periodontal regenerative therapy. This study evaluated shrinkage in rod-shaped self-assembled microtissues of human gingiva-derived cells (GC) and periodontal ligament-derived cells (PDLC) and explored the mechanisms behind it.
GC and PDLC were seeded in non-adherent agarose molds to form rod microtissues, with cells characterized by FACS. Viability was assessed by resazurin cytotoxicity assays, trypan blue exclusion, MTT, live/dead staining, and hematoxylin-eosin histology. Rod contraction was measured at 0, 2, 6, and 24 hours and compared against L-929 cells, and the role of contributing pathways in the contraction was investigated.
Key Discoveries
- Rod microtissues of gingiva and periodontal ligament cells formed in non-adherent agarose molds
- Contraction quantified at 0, 2, 6, and 24 h against L-929 fibroblast rods
- Viability confirmed by resazurin, trypan blue, MTT, live/dead, and H&E histology
